Mô tả sản phẩm: MyTaq™ DNA Polymerase Bioline
"Studying pollen-mediated gene flow means I have very limited sample volumes, MyTaq is the only polymerase I have found that can cope with this efficiently."
- Stirling University, Scotland
Features
- New generation polymerase with superior performance
- Increased sensitivity and speed
- Novel buffer system, including dNTPs and MgCl2
- Robust and high yield across a wide range of templates
Applications
- Specific amplification of complex templates
- Robust amplification of GC-rich sequences
- Routine PCR applications
- TA cloning
Description
MyTaq™ DNA Polymerase is a high performance PCR reagent that exhibits more robust amplification than other commonly used polymerases (Fig. 1), delivering very high yield over a wide range of PCR templates and making it the ideal choice for most routine assays. This new enzyme developed by Bioline is supplied with MyTaq 5x Reaction Buffer system, an advanced formulation that saves time and delivers superior results (Fig. 2). MyTaq buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations which eliminates the need for optimization.
Components
- MyTaq DNA Polymerase
- 5x MyTaq Reaction Buffer
Unit Definition
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.
Concentration
5u/µl
Storage Conditions
MyTaq DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.
Product Citations
- Waldorf, K.M., et al. PLoS One 6(12), e28972 (2011).
- Cadar, D., et al. Virus Genes doi: 10.1007/s11262-011-0650-4 (2011).
- Cadar, D., et al. Arch. Virol. doi: 10.1007/s00705-011-1125-6 (2011).
Notes
MyTaq is a trademark of Bioline.
