Mô tả sản phẩm: Bioline ISOLATE II Plant DNA Kit ( Kit Tách chiết DNA thực vật)
"Working with fungi means to accept compromises when using DNA isolation kits because there aren´t specialized kits for fungal application in most cases. The ISOLATE II Plant DNA Kit worked very well for Alternaria alternata and for unknown fungal soil isolates."
- Karlsruhe, Institute of Technology
Features
- Plant genomic DNA isolated in 30 minutes
- High-purity DNA: typical A260/A280 ratio 1.6 - 1.9
- Choice of two optimized lysis buffers
- Extra filters for clarification of lysate included
- RNase A included
Applications
Isolation of genomic DNA from:
- Isolation of genomic DNA from:
- Fresh/frozen/lyophilized plant tissue
- Dung, animal fecal, soil and compost samples
- Herbarium specimens
- Fungi
- Isolated DNA is ready for downstream applications such as PCR, real-time PCR and genotyping
Isolated DNA is ready for downstream applications such as PCR, real-time PCR and genotyping
Description
ISOLATE II Plant DNA Kit is designed for the rapid purification of genomic DNA from a variety of wet or dry plant material, including leaves, bark, roots, fruits, etc. Up to 100mg wet plant material and up to 20mg dry plant material can be processed per spin column. The protocol does not require the use of Proteinase K, which means that all components can be conveniently stored at room temperature. The plant samples are homogenized by mechanical treatment. DNA is then extracted with chaotropic salts, denaturing agents and detergents. Crude lysates are cleared in order to remove polysaccharides, contaminants and residual cellular debris. The DNA is bound to a silica membrane, washed and the pure genomic DNA is eluted.
The kit contains two optimized lysis buffers based on the established CTAB and SDS methods. RNase A is included to remove RNA.
ISOLATE II Plant DNA Kit shows excellent recovery of plant DNA with a variety of homogenization techniques (Fig. 1). High yields are obtained with every miniprep (Fig. 2).
The online product manual has protocols for purifying plant genomic DNA, fungal genomic DNA, dung, animal fecal, soil and compost samples. The isolated DNA is ready for use in downstream applications such as PCR, real-time PCR, genotyping, restriction analysis and sequencing.
Components
ISOLATE II Filters
ISOLATE II Plant Columns
Collection Tubes
Lysis Buffer PA1
Lysis Buffer PA2
Precipitation Buffer PL3
Binding Buffer PB
Wash Buffer PAW1
Wash Buffer PAW2 (Concentrate)
Elution Buffer PG
RNase A (lyophilized)
Product Manual
Bench Protocol Sheet
Storage Conditions
ISOLATE II Plant DNA Kit contents should be stored dry at room temperature. Under these conditions, the kit is stable for 12 months.
Shipping Conditions
Room temperature.
